Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34⁺ cells.

ABSTRACT:

Objectives

Stem cell factor (SCF) is considered as a commonly indispensable cytokine for proliferation of haematopoietic stem cells (HSCs), which is used in large dosages during ex vivo culture. The work presented here aimed to reduce the consumption of SCF by sustained release but still support cells proliferation and maintain the multipotency of HSCs.

Materials and methods

Stem cell factor was physically encapsulated within a hyaluronic acid/gelatin double network (HGDN) hydrogel to achieve a slow release rate. CD34⁺ cells were cultured within the SCF-loaded HGDN hydrogel for 14 days. The cell number, phenotype and functional capacity were investigated after culture.

Results

The HGDN hydrogels had desirable properties and encapsulated SCF kept being relea

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6528907 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature