MiR-181d regulates human dendritic cell maturation through NF-κB pathway.

ABSTRACT:

Objectives

MicroRNAs (miRNAs) are considered as the cellular regulators which post-transcriptionally modulate gene expression in diverse biological processes including cell development and immunity. In this study, we investigated functions of miR-181d in dendritic cells (DCs) maturation, and the underlying mechanisms were also explored.

Materials and methods

Here we did the miRNA screening in human DCs in response to lipopolysaccharides (LPS) by quantitative real-time PCR (qRT-PCR). The expressions of DCs maturation markers were measured after miRNA mimics transfections. The pharmacological inhibitors of signalling pathways were applied to examine miR-181d effect on DCs maturation by Western blot. Luciferase assay and mixed lymphocyte reaction (MLR) were also performed to re

SUBMITTER: Su XW 

PROVIDER: S-EPMC6529105 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature