Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors.

ABSTRACT: Extracellular vesicles (EVs) are membrane-surrounded structures that transmit biologically important molecules from the releasing to target cells, thus providing a novel intercellular communication mechanism. Since EVs carry their cargo in a protected form and their secretion is generally increased in tumorigenesis, EVs hold a great potential for early cancer diagnosis. By 3D culturing, we provide evidence that colorectal cancer (CRC) patient-derived organoids, representing a state-of-the-art established and essential approach for studying human CRC, is a suitable model for EV analysis. When testing the effects of major factors promoting CRC progression on EV release in the organoid model, we observed that Apc mutation, leading to uncontrolled Wnt activation and thus to tumorigenesis in th

SUBMITTER: Szvicsek Z 

PROVIDER: S-EPMC6529386 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature