Intensive formation of coccoid forms as a feature strongly associated with highly pathogenic Helicobacter pylori strains.

ABSTRACT: The variability of Helicobacter pylori morphology and the heterogeneity of virulence factors expressed by these bacteria play a key role as a driving force for adaptation to the hostile stomach environment. The aim of the study was to determine the relationship between the presence of certain genes encoding virulence factors and H. pylori morphology. One reference and 13 clinical H. pylori strains with a known virulence profile (vacA, cagA, babA2, dupA, and iceA) were used in this study. Bacteria were cultured for 1 h and 24 h in stressogenic culture conditions, i.e., serum-free BHI broths at suboptimal conditions (room temperature and atmosphere, without shaking). H. pylori cell morphology was observed by light and scanning electron microscopy. The vacA polymorphism and the cagA and babA2

SUBMITTER: Krzyzek P 

PROVIDER: S-EPMC6529389 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature