Mycofactocin Is Associated with Ethanol Metabolism in Mycobacteria.

ABSTRACT: Mycofactocin (MFT) belongs to the class of ribosomally synthesized and posttranslationally modified peptides conserved in many ActinobacteriaMycobacterium tuberculosis assimilates cholesterol during chronic infection, and its in vitro growth in the presence of cholesterol requires most of the MFT biosynthesis genes (mftA, mftB, mftC, mftD, mftE, and mftF), although the reasons for this requirement remain unclear. To identify the function of MFT, we characterized MFT biosynthesis mutants constructed in Mycobacterium smegmatis, M. marinum, and M. tuberculosis We found that the growth deficit of mft deletion mutants in medium containing cholesterol-a phenotypic basis for gene essentiality prediction-dep

SUBMITTER: Krishnamoorthy G 

PROVIDER: S-EPMC6529628 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature