Cellular landmarks of Trypanosoma brucei and Leishmania mexicana.

ABSTRACT: The kinetoplastids Trypanosoma brucei and Leishmania mexicana are eukaryotes with a highly structured cellular organisation that is reproduced with great fidelity in each generation. The pattern of signal from a fluorescently tagged protein can define the specific structure/organelle that this protein localises to, and can be extremely informative in phenotype analysis in experimental perturbations, life cycle tracking, post-genomic assays and functional analysis of organelles. Using the vast coverage of protein subcellular localisations provided by the TrypTag project, an ongoing project to determine the localisation of every protein encoded in the T. brucei genome, we have generated an inventory of reliable reference organelle markers for both parasites that combines epifluorescence imag

SUBMITTER: Halliday C 

PROVIDER: S-EPMC6529878 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature