Patterns in the longitudinal oropharyngeal microbiome evolution related to ventilator-associated pneumonia.

ABSTRACT:

Background

The aim of the study was to evaluate the composition and the temporal evolution of the oropharyngeal microbiome in antibiotic-naïve patients requiring mechanical ventilation and to gain new insights into the pathogenesis of ventilator-associated pneumonia (VAP).

Methods

Prospective, observational single-center nested case-control study. Patients with acute critical illness and anticipated duration of mechanical ventilation > 4 days were eligible. We took oropharyngeal swabs (and if available, tracheal secretions) daily, starting at the day of intubation. The microbiota was characterized by 16S rRNA high-throughput sequencing and compared between patients developing VAP versus controls.

Results

Five patients developed VAP. In three patient the causative path

SUBMITTER: Sommerstein R 

PROVIDER: S-EPMC6530040 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature