A single-cell transcriptomic atlas of the developing chicken limb.

ABSTRACT:

Background

Through precise implementation of distinct cell type specification programs, differentially regulated in both space and time, complex patterns emerge during organogenesis. Thanks to its easy experimental accessibility, the developing chicken limb has long served as a paradigm to study vertebrate pattern formation. Through decades' worth of research, we now have a firm grasp on the molecular mechanisms driving limb formation at the tissue-level. However, to elucidate the dynamic interplay between transcriptional cell type specification programs and pattern formation at its relevant cellular scale, we lack appropriately resolved molecular data at the genome-wide level. Here, making use of droplet-based single-cell RNA-sequencing, we catalogue the developmental emergence of

SUBMITTER: Feregrino C 

PROVIDER: S-EPMC6530069 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature