Development and evaluation of a milk protein transcript depletion method for differential transcriptome analysis in mammary gland tissue.

ABSTRACT:

Background

In the mammary gland transcriptome of lactating dairy cows genes encoding milk proteins are highly abundant, which can impair the detection of lowly expressed transcripts and can bias the outcome in global transcriptome analyses. Therefore, the aim of this study was to develop and evaluate a method to deplete extremely highly expressed transcripts in mRNA from lactating mammary gland tissue.

Results

Selective RNA depletion was performed by hybridization of antisense oligonucleotides targeting genes encoding the caseins (CSN1S1, CSN1S2, CSN2 and CSN3) and whey proteins (LALBA and PAEP) within total RNA followed by RNase H-mediated elimination of the respective transcripts. The effect of the RNA depletion procedure was monitored by RNA sequencing analysis comparing

SUBMITTER: Brodhagen J 

PROVIDER: S-EPMC6530097 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature