IGF-1 overexpression improves mesenchymal stem cell survival and promotes neurological recovery after spinal cord injury.

ABSTRACT:

Background

Survival and therapeutic actions of bone marrow-derived mesenchymal stem cells (BMMSCs) can be limited by the hostile microenvironment present during acute spinal cord injury (SCI). Here, we investigated whether BMMSCs overexpressing insulin-like growth factor 1 (IGF-1), a cytokine involved in neural development and injury repair, improved the therapeutic effects of BMMSCs in SCI.

Methods

Using a SCI contusion model in C57Bl/6 mice, we transplanted IGF-1 overexpressing or wild-type BMMSCs into the lesion site following SCI and evaluated cell survival, proliferation, immunomodulation, oxidative stress, myelination, and functional outcomes.

Results

BMMSC-IGF1 transplantation was associated with increased cell survival and recruitment of endogenous neural prog

SUBMITTER: Allahdadi KJ 

PROVIDER: S-EPMC6530133 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature