The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B₁.

ABSTRACT:

Background

Vitamin B₁ (V_B1) is a crucial dietary nutrient and essential cofactor for several key enzymes in the regulation of cellular and metabolic processes, and more importantly in the activation of immune system. To date, the precise role of V_B1 in Mycobacterium tuberculosis remains to be fully understood.

Results

In this study, the transcriptional and metabolic profiles of V_B1-treated Mycobacterium. bovis BCG were analyzed by RNA-sequencing and LC-MS (Liquid chromatography coupled to mass spectrometry). The selection of BCG strain was based on its common physiological features shared with M. tuberculosis. The results of cell growth assays demonstrated that V_B1 inhibited the BCG growth rate in vitro. Transcriptomic a

SUBMITTER: Song N 

PROVIDER: S-EPMC6530141 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature