Monitoring flux in signalling pathways through measurements of 4EBP1-mediated eIF4F complex assembly.

ABSTRACT:

Background

The most commonly occurring cancer mutations, including oncogenes such as MYC, Ras and PIK3C, are found in signal transductions pathways feeding into the translational machinery. A broad range of translation initiation factors are also commonly found to be either amplified or mis-regulated in tumours, including eIF4E (elongation initiation factor 4E). eIF4E is a subunit of the eIF4F protein initiation complex and required for its recruitment. Here we measure the formation of the eIF4F complex through interactions of eIF4E and eIF4G subunits, and the effect of oncogenic signalling pathways on complex formation.

Results

We developed a protein fragment complementation (PCA) assay that can accurately measure the status of the eIF4E-eIF4G interaction in cells and quant

SUBMITTER: Frosi Y 

PROVIDER: S-EPMC6530213 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature