NosL is a dedicated copper chaperone for assembly of the Cu_Z center of nitrous oxide reductase.

ABSTRACT: Nitrous oxide reductase (N₂OR) is the terminal enzyme of the denitrification pathway of soil bacteria that reduces the greenhouse gas nitrous oxide (N₂O) to dinitrogen. In addition to a binuclear Cu_A site that functions in electron transfer, the active site of N₂OR features a unique tetranuclear copper cluster bridged by inorganic sulfide, termed Cu_Z. In copper-limited environments, N₂OR fails to function, resulting in truncation of denitrification and rising levels of N₂O released by cells to the atmosphere, presenting a major environmental challenge. Here we report studies of nosL from Paracoccus denitrificans, which is part of the nos gene cluster, and encodes a putative copper binding prot

SUBMITTER: Bennett SP 

PROVIDER: S-EPMC6530538 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature