5HTR3A-driven GFP labels immature olfactory sensory neurons.

ABSTRACT: The ionotropic serotonin receptor, 5-HT₃ , is expressed by many developing neurons within the central nervous system. Since the olfactory epithelium continues to generate new olfactory sensory neurons (OSNs) throughout life, we investigated the possibility that 5-HT₃ is expressed in the adult epithelium. Using a transgenic mouse in which the promoter for the 5-HT_3a subunit drives expression of green fluorescent protein (GFP), we assessed the expression of this marker in the olfactory epithelium of adult mice. Both the native 5-HT_3a mRNA and GFP are expressed within globose basal cells of the olfactory and vomeronasal epithelium in adult mice. Whereas the 5-HT_3a mRNA disappears relatively quickly after final cell division, the GFP label

SUBMITTER: Finger TE 

PROVIDER: S-EPMC6530560 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature