Ultrathin Dual-Scale Nano- and Microporous Membranes for Vascular Transmigration Models.

ABSTRACT: Selective cellular transmigration across the microvascular endothelium regulates innate and adaptive immune responses, stem cell localization, and cancer cell metastasis. Integration of traditional microporous membranes into microfluidic vascular models permits the rapid assay of transmigration events but suffers from poor reproduction of the cell permeable basement membrane. Current microporous membranes in these systems have large nonporous regions between micropores that inhibit cell communication and nutrient exchange on the basolateral surface reducing their physiological relevance. Here, the use of 100 nm thick continuously nanoporous silicon nitride membranes as a base substrate for lithographic fabrication of 3 µm pores is presented, resulting in a highly porous (≈30%), dual-scale

SUBMITTER: Salminen AT 

PROVIDER: S-EPMC6530565 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature