Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients.

ABSTRACT: Understanding the immunological phenotype of transplant recipients is important to improve outcomes and develop new therapies. Immunophenotyping of whole peripheral blood (WPB) by flow cytometry is a rapid method to obtain large amounts of data relating to the outcomes of different transplant treatments with limited patient impact. Healthy individuals and patients with type 1 diabetes (T1D) enrolled in islet transplantation were recruited and WPB was collected. 46 fluorochrome-conjugated mouse-anti-human antibodies were used (43 of 46 antibodies were titrated). BD cytometer setup and tracking beads were used to characterize and adjust for cytometer performance. Antibody cocktails were pre-mixed <60 minutes before staining. Multicolour panels were designed based on fluorochrome brightness,

SUBMITTER: Jimenez Vera E 

PROVIDER: S-EPMC6530858 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature