Dissection of the Hydrogen Metabolism of the Enterobacterium Trabulsiella guamensis: Identification of a Formate-Dependent and Essential Formate Hydrogenlyase Complex Exhibiting Phylogenetic Similarity to Complex I.

ABSTRACT: Trabulsiella guamensis is a nonpathogenic enterobacterium that was isolated from a vacuum cleaner on the island of Guam. It has one H₂-oxidizing Hyd-2-type hydrogenase (Hyd) and encodes an H₂-evolving Hyd that is most similar to the uncharacterized Escherichia coli formate hydrogenlyase (FHL-2 _Ec ) complex. The T. guamensis FHL-2 (FHL-2 _Tg ) complex is predicted to have 5 membrane-integral and between 4 and 5 cytoplasmic subunits. We showed that the FHL-2 _Tg complex catalyzes the disproportionation of formate to CO₂ and H₂ FHL-2 _Tg has activity similar to that of the E. coli FHL-1 _Ec complex in H₂ evolution from formate

SUBMITTER: Lindenstrauß U 

PROVIDER: S-EPMC6531613 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature