Discovering metabolic disease gene interactions by correlated effects on cellular morphology.

ABSTRACT:

Objective

Impaired expansion of peripheral fat contributes to the pathogenesis of insulin resistance and Type 2 Diabetes (T2D). We aimed to identify novel disease-gene interactions during adipocyte differentiation.

Methods

Genes in disease-associated loci for T2D, adiposity and insulin resistance were ranked according to expression in human adipocytes. The top 125 genes were ablated in human pre-adipocytes via CRISPR/CAS9 and the resulting cellular phenotypes quantified during adipocyte differentiation with high-content microscopy and automated image analysis. Morphometric measurements were extracted from all images and used to construct morphologic profiles for each gene.

Results

Over 10⁷ morphometric measurements were obtained. Clustering of the morpholog

SUBMITTER: Jiao Y 

PROVIDER: S-EPMC6531784 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature