Structure and Dynamics of the Central Lipid Pool and Proteins of the Bacterial Holo-Translocon.

ABSTRACT: The bacterial Sec translocon, SecYEG, associates with accessory proteins YidC and the SecDF-YajC subcomplex to form the bacterial holo-translocon (HTL). The HTL is a dynamic and flexible protein transport machine capable of coordinating protein secretion across the membrane and efficient lateral insertion of nascent membrane proteins. It has been hypothesized that a central lipid core facilitates the controlled passage of membrane proteins into the bilayer, ensuring the efficient formation of their native state. By performing small-angle neutron scattering on protein solubilized in "match-out" deuterated detergent, we have been able to interrogate a "naked" HTL complex, with the scattering contribution of the surrounding detergent micelle rendered invisible. Such an approach has allowed th

SUBMITTER: Martin R 

PROVIDER: S-EPMC6531790 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature