Laser capture microdissection of human pancreatic islets reveals novel eQTLs associated with type 2 diabetes.

ABSTRACT:

Objective

Genome wide association studies (GWAS) for type 2 diabetes (T2D) have identified genetic loci that often localise in non-coding regions of the genome, suggesting gene regulation effects. We combined genetic and transcriptomic analysis from human islets obtained from brain-dead organ donors or surgical patients to detect expression quantitative trait loci (eQTLs) and shed light into the regulatory mechanisms of these genes.

Methods

Pancreatic islets were isolated either by laser capture microdissection (LCM) from surgical specimens of 103 metabolically phenotyped pancreatectomized patients (PPP) or by collagenase digestion of pancreas from 100 brain-dead organ donors (OD). Genotyping (> 8.7 million single nucleotide polymorphisms) and expression (> 47,000 transcript

SUBMITTER: Khamis A 

PROVIDER: S-EPMC6531807 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature