Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators.

ABSTRACT: For over 20 years, genetically encoded Ca²⁺ indicators have illuminated dynamic Ca²⁺ signaling activity in living cells and, more recently, whole organisms. We are just now beginning to understand how they work. Various fluorescence colors of these indicators have been developed, including red. Red ones are promising because longer wavelengths of light scatter less in tissue, making it possible to image deeper. They are engineered from a red fluorescent protein that is circularly permuted and fused to a Ca²⁺-sensing domain. When Ca²⁺ binds, a conformational change in the sensing domain causes a change in fluorescence. Three factors can contribute to this fluorescence change: 1) a shift in the protonation equilibrium of the chromophore, 2) a chang

SUBMITTER: Molina RS 

PROVIDER: S-EPMC6531872 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature