Attenuation of a Pathogenic Mycoplasma Strain by Modification of the obg Gene by Using Synthetic Biology Approaches.

ABSTRACT: Mycoplasma species are responsible for several economically significant livestock diseases for which there is a need for new and improved vaccines. Most of the existing mycoplasma vaccines are attenuated strains that have been empirically obtained by serial passages or by chemical mutagenesis. The recent development of synthetic biology approaches has opened the way for the engineering of live mycoplasma vaccines. Using these tools, the essential GTPase-encoding gene obg was modified directly on the Mycoplasma mycoides subsp. capri genome cloned in yeast, reproducing mutations suspected to induce a temperature-sensitive (TS⁺) phenotype. After transplantation of modified genomes into a recipient cell, the phenotype of the resulting M. mycoides s

SUBMITTER: Lartigue C 

PROVIDER: S-EPMC6531878 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature