Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker.

ABSTRACT:

Introduction

Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method.

Methods

A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite.

Results

Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succe

SUBMITTER: Shahnazi M 

PROVIDER: S-EPMC6531938 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature