Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer.

ABSTRACT: Pancreatic cancer is a highly lethal malignancy. Lack of early diagnostic markers makes timely detection of pancreatic cancer a highly challenging endeavor. Exosomes have emerged as information-rich cancer specific biomarkers. However, characterization of tumor-specific exosomes has been challenging. This study investigated the proof of principle that exosomes could be used for the detection of pancreatic cancer. Label-free analysis of exosomes purified from normal and pancreatic cancer cell lines was performed using surface enhanced Raman Spectroscopy (SERS) and principal component differential function analysis (PC-DFA), to identify tumor-specific spectral signatures. This method differentiated exosomes originating from pancreatic cancer or normal pancreatic epithelial cell lines with 90

SUBMITTER: Carmicheal J 

PROVIDER: S-EPMC6532067 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature