Genetic and biological characteristics of avian influenza virus subtype H1N8 in environments related to live poultry markets in China.

ABSTRACT:

Background

Since 2008, avian influenza surveillance in poultry-related environments has been conducted annually in China. Samples have been collected from environments including live poultry markets, wild bird habitats, slaughterhouses, and poultry farms. Multiple subtypes of avian influenza virus have been identified based on environmental surveillance, and an H1N8 virus was isolated from the drinking water of a live poultry market.

Methods

Virus isolation was performed by inoculating influenza A-positive specimens into embryonated chicken eggs. Next-generation sequencing was used for whole-genome sequencing. A solid-phase binding assay was performed to test the virus receptor binding specificity. Trypsin dependence plaque formation assays and intravenous pathogenicity inde

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6532177 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature