Enhancement of tanshinone production in Salvia miltiorrhiza hairy root cultures by metabolic engineering.

ABSTRACT:

Background

Tanshinones are diterpenoid compounds that are used to treat cardiovascular diseases. As current extraction methods for tanshinones are inefficient, there is a pressing need to improve the production of these bioactive compounds to meet increasing demand.

Results

Overexpression of SmMDS (2-c-methyl-d-erythritol 2,4-cyclodiphosphate synthase, a tanshinone biosynthesis gene) in transgenic Salvia miltiorrhiza hairy roots significantly increased the tanshinone yield compared to the control, and total tanshinone content in SmMDS-overexpressing lines increased after elicitor treatment. Total tanshinones increased to 2.5, 2.3, and 3.2 mg/g DW (dry weight) following treatment with Ag⁺, YE (yeast extract), and MJ (methyl jasmonate), respecti

SUBMITTER: Wei T 

PROVIDER: S-EPMC6532201 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature