Biodiversity and phylogeny of novel Trichoderma isolates from mangrove sediments and potential of biocontrol against Fusarium strains.

ABSTRACT:

Background

Studies carried out with novel 13 strains of Trichoderma, isolated from mangrove sediments (PE, Brazil) using morphophysiological and molecular characterization, followed evaluation of biocontrol using Fusarium strains isolated from Caatinga soil (PE, Brazil). Trichoderma strains were characterized by polyphasic taxonomic approach, and the extracted DNA was amplified with primers ITS 1 and 4, and sequenced. The biocontrol evaluation was conducted at 24 and 48 h of growth intervals by Tukey test, with a significance of 5%. Antibiosis tests were assessed in vitro by dual plate and partition plate techniques against Fusarium strains.

Results

Trichoderma molecular identification, sequences of 500 bp were amplified, deposited into GenBank, and used for phylogenetic ana

SUBMITTER: Filizola PRB 

PROVIDER: S-EPMC6532204 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature