Integrated high-throughput analysis identifies super enhancers associated with chemoresistance in SCLC.

ABSTRACT: BACKGROUND:Chemoresistance is a primary clinical challenge for the management of small cell lung cancer. Additionally, transcriptional regulation by super enhancer (SE) has an important role in tumor evolution. The functions of SEs, a key class of noncoding DNA cis-regulatory elements, have been the subject of many recent studies in the field of cancer research. METHODS:In this study, using chromatin immunoprecipitation-sequencing and RNA-sequencing (RNA-seq), we aimed to identify SEs associated with chemoresistance from H69AR cells. Through integrated bioinformatics analysis of the MEME chip, we predicted the master transcriptional factors (TFs) binding to SE sites and verified the relationships between TFs of SEs and drug resistance by RNA interference, cell counting kit 8 assays, quanti

SUBMITTER: Bao J 

PROVIDER: S-EPMC6532255 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature