Multilocus sequence typing analysis of Candida africana from vulvovaginal candidiasis.

ABSTRACT:

Background

Candida africana is distributed worldwide and colonized in human genitalia and cause mainly vulvovaginal candidiasis (VVC). We report the multilocus sequence typing (MLST) analysis of C. africana from VVC.

Methods

MLST analysis of 43 strains of C. africana, which were isolated from vaginal specimens of patients with VVC, was performed. The enzymatic activity of phospholipase, esterase and haemolysis enzyme production was evaluated.The level of virulent genes and resistant genes mRNA expression was determined by using real-time PCR. Antifungal susceptibilities of the isolates were assayed by using the broth microdilution method. The statistical of the results was determined by the T test and Pearson chi-squared test.

Results

The MLST analysis revealed a subs

SUBMITTER: Zhu YX 

PROVIDER: S-EPMC6532261 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature