Acute gastroenteritis associated with Rotavirus A among children less than 5 years of age in Nepal.

ABSTRACT:

Background

Rotavirus gastroenteritis is a major public health problem in Nepal. This study was conducted to obtain information associated with Rotavirus gastroenteritis and to perform genotyping of Rotavirus A.

Methods

Hospital based cross sectional study was conducted from January to December 2017 among children less than 5 years of age attending Kanti Children's Hospital and Tribhuvan University Teaching Hospital. Rotavirus A antigen detection was performed by Enzyme Linked Immunosorbent Assay (ELISA) using ProSpecT Rotavirus Microplate Assay. Rotavirus A positive strains were further confirmed by genotyping using Reverse-Transcription Polymerase Chain Reaction (RT-PCR).

Results

A total of 1074 stool samples were collected, of them 770 were hospitalized, and 304 wer

SUBMITTER: Shrestha S 

PROVIDER: S-EPMC6532269 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature