Induction of a Spindle-Assembly-Competent M Phase in Xenopus Egg Extracts.

ABSTRACT: Normal mitotic spindle assembly is a prerequisite for faithful chromosome segregation and unperturbed cell-cycle progression. Precise functioning of the spindle machinery relies on conserved architectural features, such as focused poles, chromosome alignment at the metaphase plate, and proper spindle length. These morphological requirements can be achieved only within a compositionally distinct cytoplasm that results from cell-cycle-dependent regulation of specific protein levels and specific post-translational modifications. Here, we used cell-free extracts derived from Xenopus laevis eggs to recapitulate different phases of the cell cycle in vitro and to determine which components are required to render interphase cytoplasm spindle-assembly competent in the absence of protein translation

SUBMITTER: Bisht JS 

PROVIDER: S-EPMC6532407 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature