Fatty Acid Binding to Human Serum Albumin in Blood Serum Characterized by EPR Spectroscopy.

ABSTRACT: One of the functions of Human Serum Albumin (HSA) is binding and transport of fatty acids. This ability could be altered by the presence of several blood components such as toxins or peptides - which in turn alters the functionality of the protein. We aim at characterizing HSA and its fatty acid binding in native serum environment. Native ligand binding and deviations from normal function can be monitored by electron paramagnetic resonance (EPR) spectroscopy using spin labeled fatty acids (FAs). Blood serum from healthy individuals is used to examine healthy HSA in its natural physiological conditions at different loading ratios of protein to FAs. Among the EPR spectroscopic parameters (like hyperfine coupling, line shape, rotational correlation time and population of different binding sit

SUBMITTER: Haeri HH 

PROVIDER: S-EPMC6532450 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature