Trabecular Meshwork TREK-1 Channels Function as Polymodal Integrators of Pressure and pH.

ABSTRACT:

Purpose

The concentration of protons in the aqueous humor (AH) of the vertebrate eye is maintained close to blood pH; however, pathologic conditions and surgery may shift it by orders of magnitude. We investigated whether and how changes in extra- and intracellular pH affect the physiology and function of trabecular meshwork (TM) cells that regulate AH outflow.

Methods

Electrophysiology, in conjunction with pharmacology, gene knockdown, and optical recording, was used to track the pH dependence of transmembrane currents and mechanotransduction in primary and immortalized human TM cells.

Results

Extracellular acidification depolarized the resting membrane potential by inhibiting an outward K+-mediated current, whereas alkalinization hyperpolarized the cells and augment

SUBMITTER: Yarishkin O 

PROVIDER: S-EPMC6532698 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature