Epigenetic evolution and lineage histories of chronic lymphocytic leukaemia.

ABSTRACT: Genetic and epigenetic intra-tumoral heterogeneity cooperate to shape the evolutionary course of cancer¹. Chronic lymphocytic leukaemia (CLL) is a highly informative model for cancer evolution as it undergoes substantial genetic diversification and evolution after therapy^2,3. The CLL epigenome is also an important disease-defining feature^4,5, and growing populations of cells in CLL diversify by stochastic changes in DNA methylation known as epimutations⁶. However, previous studies using bulk sequencing methods to analyse the patterns of DNA methylation were unable to determine whether epimutations affect CLL populations homogeneously. Here, to measure the epimutation rate at single-cell resolution, we applied multiplexed single-cell reduced-repre

SUBMITTER: Gaiti F 

PROVIDER: S-EPMC6533116 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature