Noncoded Amino Acids in de Novo Metalloprotein Design: Controlling Coordination Number and Catalysis.

ABSTRACT: The relationship between structure and function has long been one of the major points of investigation in Biophysics. Understanding how much, or how little, of a protein's often complicated structure is necessary for its function can lead to directed therapeutic strategies and would allow one to design proteins for specific desired functions. Studying protein function by de novo design builds the functionality from the ground up in a completely unrelated and noncoded protein scaffold. Our lab has used this strategy to study heavy and transition metal binding within the TRI family of three stranded coiled coil (3SCC) constructs to understand coordination geometry and metalloenzyme catalytic control within a protein environment. These peptides contain hydrophobic layers within the interior o

SUBMITTER: Koebke KJ 

PROVIDER: S-EPMC6533121 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature