IRF3 and IRF7 mediate neovascularization via inflammatory cytokines.

ABSTRACT:

Objective

To elucidate the role of interferon regulatory factor (IRF)3 and IRF7 in neovascularization.

Methods

Unilateral hind limb ischaemia was induced in Irf3^-/- , Irf7^-/- and C57BL/6 mice by ligation of the left common femoral artery. Post-ischaemic blood flow recovery in the paw was measured with laser Doppler perfusion imaging. Soleus, adductor and gastrocnemius muscles were harvested to investigate angiogenesis and arteriogenesis and inflammation.

Results

Post-ischaemic blood flow recovery was decreased in Irf3^-/- and Irf7^-/- mice compared to C57BL/6 mice at all time points up to and including sacrifice, 28 days after surgery (t28). This was supported by a decrease in angiogenesis and arteriogenesis in soleus and addu

SUBMITTER: Simons KH 

PROVIDER: S-EPMC6533520 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature