Interobserver variability and accuracy of p16/Ki-67 dual immunocytochemical staining on conventional cervical smears.

ABSTRACT:

Background

p16/Ki-67 dual immunocytochemical staining (DS) has been proven as a sensitive and specific test for triage of HPV positive women with good reproducibility and accuracy. However, implementation of the test into an organized screening program (OSP) is not easy. The aims of this study were to compare the performance and agreement of DS results among three Slovenian cytopathological laboratories involved in the national OSP, and to define cases where staining results can be difficult to interpret.

Methods

Cervical smears were obtained for DS from 129 women referred to colposcopy. Smears were evaluated blindly in three laboratories by a cytotechnologist and a cytopathologist after initial training. Results were positive, suspicious, negative or inadequate. Five charac

SUBMITTER: Kloboves Prevodnik V 

PROVIDER: S-EPMC6533697 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature