Exploiting orthology and de novo transcriptome assembly to refine target sequence information.

ABSTRACT:

Background

The ability to generate recombinant drug target proteins is important for drug discovery research as it facilitates the investigation of drug-target-interactions in vitro. To accomplish this, the target's exact protein sequence is required. Public databases, such as Ensembl, UniProt and RefSeq, are extensive protein and nucleotide sequence repositories. However, many sequences for non-human organisms are predicted by computational pipelines and may thus be incomplete or incorrect. This could lead to misinterpreted experimental outcomes due to gaps or errors in orthologous drug target sequences. Transcriptome analysis by RNA-Seq has been established as a standard method for gene expression analysis. Apart from this common application, paired-end RNA-Seq data can also be u

SUBMITTER: Sollner JF 

PROVIDER: S-EPMC6533699 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature