Wnt Signaling Mediates the Aging-Induced Differentiation Impairment of Intestinal Stem Cells.

ABSTRACT: Stem cell aging underlies aging-associated disorders, such as steeply increased incidences of tumors and impaired regeneration capacity upon stress. However, whether and how the intestinal stem cells age remains largely unknown. Here we show that intestinal stem cells derived from 24-month-old mice hardly form typical organoids with crypt-villus structures, but rather mainly form big, rounded cysts devoid of differentiated cell types, which mimics the culturing of heterozygous APC-deficient cells from the APC^min mouse line. Further analysis showed that cultured crypts derived from aged mice exhibited reduced expression levels of differentiation genes and higher expression of Wnt target genes. Lowering the concentration of R-spondin-1 in the culture system significantly reduced f

SUBMITTER: Cui H 

PROVIDER: S-EPMC6534527 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature