Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing.

ABSTRACT: Infectious diseases caused by bacterial pathogens remain one of the most common causes of morbidity and mortality worldwide. Rapid microbiological analysis is required for prompt treatment of bacterial infections and to facilitate antibiotic stewardship. This study reports an adaptable microfluidic system for rapid pathogen classification and antimicrobial susceptibility testing (AST) at the single-cell level. By incorporating tunable microfluidic valves along with real-time optical detection, bacteria can be trapped and classified according to their physical shape and size for pathogen classification. By monitoring their growth in the presence of antibiotics at the single-cell level, antimicrobial susceptibility of the bacteria can be determined in as little as 30 minutes compared with da

SUBMITTER: Li H 

PROVIDER: S-EPMC6535027 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature