Expression Profiling of Inflammatory and Immunological Genes in Collagenous Colitis.

ABSTRACT:

Background

Collagenous colitis [CC] is a common idiopathic cause of chronic watery diarrhoea. We investigated its pathogenesis by means of gene expression analysis.

Methods

We analysed the expression of genes implicated in immunological and inflammatory pathways in paired colonic biopsies of histologically involved and uninvolved mucosa from five patients with histologically patchy CC, in pooled colonic biopsies of eight other patients with diffuse CC, and in pooled biopsies of eight normal controls. Analyses were performed with the Nanostring nCounter system. Expression ratios were generated and confirmed by quantitative reverse transcription PCR.

Results

CC mucosa was characterized by enhanced expression of nitric oxide synthase 2; of matrix metalloproteinases 3 and

SUBMITTER: Liu Q 

PROVIDER: S-EPMC6535503 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature