Bioprinting of three-dimensional dentin-pulp complex with local differentiation of human dental pulp stem cells.

ABSTRACT: Numerous approaches have been introduced to regenerate artificial dental tissues. However, conventional approaches are limited when producing a construct with three-dimensional patient-specific shapes and compositions of heterogeneous dental tissue. In this research, bioprinting technology was applied to produce a three-dimensional dentin-pulp complex with patient-specific shapes by inducing localized differentiation of human dental pulp stem cells within a single structure. A fibrin-based bio-ink was designed for bioprinting with the human dental pulp stem cells. The effects of fibrinogen concentration within the bio-ink were investigated in terms of printability, human dental pulp stem cell compatibility, and differentiation. The results show that micro-patterns with human dental pulp st

SUBMITTER: Han J 

PROVIDER: S-EPMC6535759 | biostudies-literature | 2019 Jan-Dec

REPOSITORIES: biostudies-literature