Temperature-Controlled Liquid-Liquid Phase Separation of Disordered Proteins.

ABSTRACT: The liquid-liquid phase separation (LLPS) of intrinsically disordered proteins (IDPs) is a commonly observed phenomenon within the cell, and such condensates are also highly attractive for applications in biomaterials and drug delivery. A better understanding of the sequence-dependent thermoresponsive behavior is of immense interest as it will aid in the design of protein sequences with desirable properties and in the understanding of cellular response to heat stress. In this work, we use a transferable coarse-grained model to directly probe the sequence-dependent thermoresponsive phase behavior of IDPs. To achieve this goal, we develop a unique knowledge-based amino acid potential that accounts for the temperature-dependent effects on solvent-mediated interactions for different types of a

SUBMITTER: Dignon GL 

PROVIDER: S-EPMC6535772 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature