Laboratory and Clinical Evaluation of DNA Microarray for the Detection of Carbapenemase Genes in Gram-Negative Bacteria from Hospitalized Patients.

ABSTRACT:

Background

The prevalence of a variety of carbapenemases in Gram-negative bacteria (GNB) has posed a global threat on clinical control and management. Monitoring and controlling the carbapenemase-producing GNB became imperative tasks for many healthcare centers. The aim of this study was to develop a high-throughput, specific, sensitive, and rapid DNA microarray-based method for the diagnosis, phenotypic confirmation, and molecular epidemiological study of carbapenemase genes.

Methods

We targeted a panel of eight carbapenemase genes, including bla _KPC, bla _NDM-1, bla _OXA-23, bla _OXA-48, bla _OXA-51, bla _IMP, bla _VIM, and bla _DIM for d

SUBMITTER: Song Y 

PROVIDER: S-EPMC6535891 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature