Distinct degenerative phenotype of articular cartilage from knees with meniscus tear compared to knees with osteoarthritis.

ABSTRACT:

Objective

To compare the transcriptome of articular cartilage from knees with meniscus tears to knees with end-stage osteoarthritis (OA).

Design

Articular cartilage was collected from the non-weight bearing medial intercondylar notch of knees undergoing arthroscopic partial meniscectomy (APM; N = 10, 49.7 ± 10.8 years, 50% females) for isolated medial meniscus tears and knees undergoing total knee arthroplasty (TKA; N = 10, 66.0 ± 7.6 years, 70% females) due to end-stage OA. Ribonucleic acid (RNA) preparation was subjected to SurePrint G3 human 8 × 60K RNA microarrays to probe differentially expressed transcripts followed by computational exploration of underlying biological processes. Real-time polymerase chain reaction amplification was performed on selected transcripts to

SUBMITTER: Rai MF 

PROVIDER: S-EPMC6536326 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature