Characterization of Laser Gold Nanowarming: A Platform for Millimeter-Scale Cryopreservation.

ABSTRACT: Preventing ice formation during cryopreservation by vitrification has led to the successful storage and banking of numerous cellular- and tissue-based biomaterials. In their breakthrough work, Peter Mazur's group achieved over 90% survival by using a laser warming technique for 100 μm mice oocytes that were cooled in 0.1 μL droplets with 2.3 M CPA and extracellularly loaded India ink (laser absorber). Laser warming can provide rapid and uniform warming rates to "outrun" damaging ice crystal growth. Here we generalize Mazur's technique for microliter-sized droplets using laser nanowarming to rewarm millimeter-scale biomaterials when loaded extracellularly and/or intracellularly with biocompatible 1064 nm resonant gold nanoparticles. First, we show that droplets containing low-concentration

SUBMITTER: Khosla K 

PROVIDER: S-EPMC6536355 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature