3-dimensional visualization of implant-tissue interface with the polyethylene glycol associated solvent system tissue clearing method.

ABSTRACT:

Objectives

Dental implants are major treatment options for restoring teeth loss. Biological processes at the implant-tissue interface are critical for implant osseointegration. Superior mechanical properties of the implant constitute a major challenge for traditional histological techniques. It is imperative to develop new technique to investigate the implant-tissue interface.

Materials and methods

Our laboratory developed the polyethylene glycol (PEG)-associated solvent system (PEGASOS) tissue clearing method. By immersing samples into various chemical substances, bones and teeth could be turned to transparent with intact internal structures and endogenous fluorescence being preserved. We combined the PEGASOS tissue clearing method with transgenic mouse line and other label

SUBMITTER: Yi Y 

PROVIDER: S-EPMC6536405 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature