Characterization of porcine extraembryonic endoderm cells.

ABSTRACT:

Objectives

To date, many efforts have been made to establish porcine embryonic stem (pES) cells without success. Extraembryonic endoderm (XEN) cells can self-renew and differentiate into the visceral endoderm and parietal endoderm. XEN cells are derived from the primitive endoderm of the inner cell mass of blastocysts and may be an intermediate state in cell reprogramming.

Materials and methods

Porcine XEN cells (pXENCs) were generated from porcine pluripotent stem cells (pPSCs) and were characterized by RNA sequencing and immunofluorescence analyses. The developmental potential of pXENCs was investigated in chimeric mouse embryos.

Results

Porcine XEN cells derived from porcine pPSCs were successfully expanded in N2B27 medium supplemented with bFGF for least 30 passag

SUBMITTER: Shen QY 

PROVIDER: S-EPMC6536407 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature