Lic regulates JNK-mediated cell death in Drosophila.

ABSTRACT:

Objectives

The evolutionary conserved JNK pathway plays crucial role in cell death, yet factors that modulate this signalling have not been fully disclosed. In this study, we aim to identify additional factors that regulate JNK signalling in cell death, and characterize the underlying mechanisms.

Materials and methods

Drosophila were raised on standard media, and cross was carried out at 25°C. The Gal4/UAS system was used to express proteins or RNAi in a specific temporal and spatial pattern. Gene expression was revealed by GFP fluorescence, X-gal staining or immunostaining of 3rd instar larval eye and wing discs. Cell death was visualized by acridine orange (AO) staining. Images of fly eyes and wings were taken by OLYMPUS microscopes.

Results

We found that licorne (l

SUBMITTER: Sun Y 

PROVIDER: S-EPMC6536442 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature