Screening of MSI detection loci and their heterogeneity in East Asian colorectal cancer patients.

ABSTRACT:

Objective

This study aims to screen the MSI detection loci suitable for the East Asian colorectal cancer patients. and explore its intratumoral heterogeneity.

Methods

A total of 271 pathological tissues specimens of colorectal cancer were collected. The MSI status was detected using different PCR reagent kits with different detection loci. Then, the results were compared with the immunohistochemical (IHC) staining results. Microdissection of pathological tissues specimens detected to be MSI-H was performed to examine whether there was intratumoral heterogeneity of MSI status.

Results

Thirty-nine out of 271 cases were dMMR. dMMR occurred mostly in patients with right-hemi colon cancer (P < 0.0001). Compared with dMMR patients, the clinical stages of pMMR patients were

SUBMITTER: Bai W 

PROVIDER: S-EPMC6536949 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature